Regeneration of Ectomycorrhizal Fungal Isolates Following Deep Freezer Storage
نویسندگان
چکیده
Mycelial growth and survival ratio of ectomycorrhizal fungi were determined after storage at -70℃ for 1, 3, or 6 mon. Seventeen of 23 ectomycorrhizal fungi did not survive after storage for more than 6 mon, whereas Cenococcum geophilum, Lepista nuda, and some species of Rhizopogon and Suillus did survive.
منابع مشابه
Long-term preservation of fungal isolates in commercially prepared cryogenic microbank vials.
Since 1994, 6,198 yeasts and 391 molds belonging to 25 and 37 species, respectively, were stored in Microbank cryogenic vials at >/=-130 degrees C in liquid nitrogen and at -70 degrees C in a freezer. All of the isolates, with the exception of 45 yeasts and 15 dermatophytes, were recovered from both storage temperatures. Good reproducibility was demonstrated for amphotericin B, fluconazole, and...
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